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sticky-end fill-in

Andrea Hansen webmaster at bioinformatik.de
Sun Jan 9 05:45:26 EST 2000


kurbanic at uoguelph.ca (Kevin Urbanic) writes:

> I am trying to blunt-end a piece of plasmid DNA with sticky ends.  Mung 
> Bean is not working and I would like to try something else.
> 
> Can anybody recommend the appropriate enzyme that will fill in the 
> single-stranded ends of a piece of plasmid DNA with sticky ends?  

Whats about Klenow? We use this enzyme to make sticky ends blunt.

Andrea

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