kurbanic at uoguelph.ca (Kevin Urbanic) writes:
> I am trying to blunt-end a piece of plasmid DNA with sticky ends. Mung
> Bean is not working and I would like to try something else.
>> Can anybody recommend the appropriate enzyme that will fill in the
> single-stranded ends of a piece of plasmid DNA with sticky ends?
Whats about Klenow? We use this enzyme to make sticky ends blunt.
Andrea
--
Institut fuer Botanik III
Heinrich-Heine-Universitaet
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