Transfection!

Shean skim7 at po-box.mcgill.ca
Mon Jan 10 00:28:50 EST 2000


Hi there!

I am working with pcDNA3/1His vector and trying to transfect into
COS-7 and HeLa cells.

When I used the LacZ control vector(1 ug), the transfection efficiency
was extremely high with Gibco Lipofectamine reagent but my protein was
hardly detectable.  I know it DOES express in the cell.  

If you have an experience with this vector and cell lines for
transfection, can you share your protocol with me?

Thank you in advance.




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