hybridization parameters for non-specific probe

Chihara chiharaNOchSPAM at usfca.edu.invalid
Mon Jan 10 15:54:03 EST 2000


I want to screen Drosopila DNA with a frog or fungal probe. Don't have
much idea of what the homology might be, although a Blast search finds
my EST in the genes.  Am trying random temps but would like some of
idea of what has worked in similar situations.  Any help out there?


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