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sticky-end fill-in

Jens Tornoe jt at neurosearch.dk
Mon Jan 10 16:05:52 EST 2000

I always use Pfu after having been annoyed with Klenow several times. You
will just have to remember to incubate @ 72 C, then.


----- Original Message -----
From: Kevin Urbanic <kurbanic at uoguelph.ca>
Newsgroups: bionet.molbio.methds-reagnts
Sent: Tuesday, January 04, 2000 5:56 PM
Subject: sticky-end fill-in

> I am trying to blunt-end a piece of plasmid DNA with sticky ends.  Mung
> Bean is not working and I would like to try something else.
> Can anybody recommend the appropriate enzyme that will fill in the
> single-stranded ends of a piece of plasmid DNA with sticky ends?
> Thanks,
> Kevin Urbanic

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