I always use Pfu after having been annoyed with Klenow several times. You
will just have to remember to incubate @ 72 C, then.
----- Original Message -----
From: Kevin Urbanic <kurbanic at uoguelph.ca>
Sent: Tuesday, January 04, 2000 5:56 PM
Subject: sticky-end fill-in
> I am trying to blunt-end a piece of plasmid DNA with sticky ends. Mung
> Bean is not working and I would like to try something else.
>>> Can anybody recommend the appropriate enzyme that will fill in the
> single-stranded ends of a piece of plasmid DNA with sticky ends?
> Kevin Urbanic