apoptosis

Bernard Murray, PhD spam at 127.0.0.1
Mon Jan 10 23:55:48 EST 2000


In article <3878B4BA.CB270188 at hotmail.com>, mpj_de_winther at hotmail.com wrote:

> I would like to quantify apoptosis in murine macrophages cultured on
> slides. What would be the easiest method (without lifting/FACSing the
> cells)?

What stage of apoptosis will the cells be in?
If you have access to a fluorescence microscope one of the
selective stains would probably be the easiest way.
If not then Klenow or Tdt labelling would give you
some idea.

   Bernard

-- 
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF




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