Henk Veldman wrote:
>> Belscotia schreef:
>> > Hi there
> > I used to use NP-40 for solubilizing my protein extracts, but now use
> > IGEPAL-CA 630 and everything still seems OK. I don't know about the
> > Bradford assay but BIO-RAD do a protein determination kit which works
> > well in the presence of many different detergents (according to bio-rad
> > anyway!) I have used this kit in the presence of 0.5% NP-40 and 0.5%
> > deoxycholate with good results.
> > I am NOT employed/sponsered by BIO-RAD!
> >
> > bye
> >
> >
>> Bio-Rad sells two assays:
> One is a Bradford variant. It is the more sensitive but is incompatible with
> detergents, dyes or strong alkali.
> The other (they call it DC assay) is a Lowry variant. It is compatible with most
> detergents, but not with thiols (it can, however, be made compatible with
> thiols).
Hi Henk,
How can it be made compatible with thiols?
> Hope this helps.
>> --
>> H. Veldman
> Laboratory for Experimental Neurology (NMZ)
> Room G 02.320
> University Medical Center Utrecht (AZU)
> P.O. Box 85500
> 3508 GA UTRECHT
> The Netherlands
> Tel: +31 30 2507565
> Fax: +31 30 2542100
> E-mail: H.Veldman at lab.azu.nl
--
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Dr. Fernando Rodríguez Pascual
Department of Pharmacology
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Obere Zahlbacher Str. 67
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Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
Fax. +49 (6131) 39 3 66 11
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