IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Nonidet P40 and Bradford

Dr. Fernando Rodriguez-Pascual frodrigu at mail.uni-mainz.de
Tue Jan 11 09:17:26 EST 2000


Henk Veldman wrote:
> 
> Belscotia schreef:
> 
> > Hi there
> > I used to use NP-40 for solubilizing my protein extracts, but now use
> > IGEPAL-CA 630 and everything still seems OK. I don't know about the
> > Bradford assay but BIO-RAD do a protein determination kit which works
> > well in the presence of many different detergents (according to bio-rad
> > anyway!) I have used this kit in the presence of 0.5% NP-40 and 0.5%
> > deoxycholate with good results.
> > I am NOT employed/sponsered by BIO-RAD!
> >
> > bye
> >
> >
> 
> Bio-Rad sells two assays:
> One is a Bradford variant. It is the more sensitive but is incompatible with
> detergents, dyes or strong alkali.
> The other (they call it DC assay) is a Lowry variant. It is compatible with most
> detergents, but not with thiols (it can, however,  be made compatible with
> thiols).

Hi Henk,

How can it be made compatible with thiols?

> Hope this helps.
> 
> --
> 
> H. Veldman
> Laboratory for Experimental Neurology (NMZ)
> Room G 02.320
> University Medical Center Utrecht (AZU)
> P.O. Box 85500
> 3508 GA  UTRECHT
> The Netherlands
> Tel: +31 30 2507565
> Fax: +31 30 2542100
> E-mail: H.Veldman at lab.azu.nl

-- 
#############################################
Dr. Fernando Rodríguez Pascual
Department of Pharmacology
Johannes Gutenberg University
Obere Zahlbacher Str. 67
D-55101 Mainz, Germany
Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
Fax. +49 (6131) 39 3 66 11
email: frodrigu at mail.uni-mainz.de
#############################################




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net