Sv: DNA isolation

guest hlindeberg at odont.au.dk
Tue Jan 11 10:17:16 EST 2000


Hi, Kelvin - if it's just for PCR, try boiling: Pick up a single colony,
suspend it in plain water and boil it for 10 minuts. Prepare a 0.9x
master-PCR-mix and ad 10 1.0x from your boiled bacteas - quick, dirty and it
somtimes works *S*
Henning


Chong Wai Yin( Zhang Weixian) <wychong at singnet.com.sg> skrev i en
nyhedsmeddelelse:854j6n$600$1 at violet.singnet.com.sg...
> Hi, does anyone know of any fast, cheap and dirty method to extract DNA
from
> a Gram positive bacteria? Thanks.
>
> Kelvin.
>
>






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