IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Nonidet P40 and Bradford

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Tue Jan 11 10:11:10 EST 2000


Fernando, 

Including the detergent into the standard 
solutions reduces the errors. 

If this doesn't work with your system, you could precipitate the 
proteins in your sample (e.g. with TCA or try a "reverse" 
phenol/chloroform extraction and keep the pellet) and wash out the 
detergent.

Wolfgang


> From:          "Dr. Fernando Rodriguez-Pascual" <frodrigu at mail.uni-mainz.de>
> Subject:       Nonidet P40 and Bradford
> Date:          Mon, 10 Jan 2000 15:13:24 +0200
> Organization:  Johannes Gutenberg-Universitaet Mainz, Germany
> Reply-to:      frodrigu at mail.uni-mainz.de
> To:            methods at hgmp.mrc.ac.uk

> Hi all,
> 
> Have any of you experienced problems in performing protein
> determinations by Bradford using extracts containing 0.5% Nonidet
> P40. I mean, whether it is compatible with the Bradford reagent and
> up to which concentration. In former times I used to have no
> problems for that but now I find that anyhow it affects the assay.
> By the way, Sigma is not longer purchasing Nonidet P40, but a
> "chemically indistinguible from it" compound (as Sigma) called
> IGEPAL-CA 630. Do you know if it is really the same? Have you found
> problems by using this new nonionic detergent?
> 
> Chao
> 
> Fernando
> -- 
> #############################################
> Dr. Fernando Rodríguez Pascual
> Department of Pharmacology
> Johannes Gutenberg University
> Obere Zahlbacher Str. 67
> D-55101 Mainz, Germany
> Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
> Fax. +49 (6131) 39 3 66 11
> email: frodrigu at mail.uni-mainz.de
> #############################################

Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
-----
*unsolicited mail is *NOT* appreciated, usual disclaimers apply 
-----                         
---




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net