Southern probes: cDNA/genomic preference??

Michael Tuvim mtuvin at bcm.tmc.edu
Tue Jan 11 11:50:24 EST 2000


 Southern blot question:
Does anybody know how much better a genomic fragment will work as a
probe then a cDNA piece?
In other words: how important is continuity in hybridization?
Obviously, if we have a cDNA probe of 500bp it should hybridase
stronger with a one-exon gene then with a  hundred-exons gene.
What would be a reasonable UNINTERRUPTED hybridization length over
which it shouldn't matter any more?
Thank ya'll.
Michael Tuvim
--
A lie isn't a lie if it is in reply to a question
that the questioner has no right to ask.
                                   Archie Goodwin


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