Southern probes: cDNA/genomic preference??
mtuvin at bcm.tmc.edu
Tue Jan 11 11:50:24 EST 2000
Southern blot question:
Does anybody know how much better a genomic fragment will work as a
probe then a cDNA piece?
In other words: how important is continuity in hybridization?
Obviously, if we have a cDNA probe of 500bp it should hybridase
stronger with a one-exon gene then with a hundred-exons gene.
What would be a reasonable UNINTERRUPTED hybridization length over
which it shouldn't matter any more?
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