pH of PCR

Colin Rasmussen colin at pombe.usask.ca
Tue Jan 11 12:14:39 EST 2000



Thomas Pfitzner wrote:

> Hi,
> has anybody observed a pH shift up to 14 after a PCR amplification ?
>
> Thomas Pfitzner

I've never checked the pH of the sample afterwards.  Why did you ?
Plus, it's impossible for it to get to 14 unless you add significant
amounts of OH.

Colin






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