PCR using DNA isolated from human plasma

Manuel Ruiz Rejon mrejon at goliat.ugr.es
Wed Jan 12 04:46:35 EST 2000


Hi,

Has anyone got a protocol for amplifying human DNA obtained from serum
(plasma) in order to analyze individual genes?.
I am trying to analyze by means of PCR the ACE-DCP1 gene using DNA from
plasma, but I have many problems (not in obtaining DNA but for the
amplification of the gene by PCR). I had not these troubles with whole
blood. Any suggestions?

Trini.





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