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Nonidet P40 and Bradford

Li Jingsong medp7095 at nus.edu.sg
Wed Jan 12 08:33:03 EST 2000


 You may try BioRad Detergent Compatible Protein assay Kit
 
-----Original Message-----
From: Dr. Fernando Rodriguez-Pascual
Posted At: 1/10/00 9:13 PM
Conversation: Nonidet P40 and Bradford
Subject: Nonidet P40 and Bradford

Hi all,

Have any of you experienced problems in performing protein
determinations by Bradford using extracts containing 0.5% Nonidet P40. I
mean, whether it is compatible with the Bradford reagent and up to which
concentration. In former times I used to have no problems for that but
now I find that anyhow it affects the assay.
By the way, Sigma is not longer purchasing Nonidet P40, but a
"chemically indistinguible from it" compound (as Sigma) called IGEPAL-CA
630. Do you know if it is really the same? Have you found problems by
using this new nonionic detergent?

Chao

Fernando
-- 
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Dr. Fernando Rodríguez Pascual
Department of Pharmacology
Johannes Gutenberg University
Obere Zahlbacher Str. 67
D-55101 Mainz, Germany
Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
Fax. +49 (6131) 39 3 66 11
email: frodrigu at mail.uni-mainz.de
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