Dear readers,
I have cloned the cDNA encoding for a new protein (letÿffffc3ÿffffa2ÿffffe2ÿffff82ÿffffacÿffffe2ÿffff84ÿffffa2s
cal
l it
protein ÿffffc3ÿffffa2ÿffffe2ÿffff82ÿffffacÿffffc5ÿffff93Aÿffffc3ÿffffa2ÿffffe2ÿffff82ÿffffacÿffffc2ÿffff9d) and I donÿffffc3ÿffffa2ÿffffe2ÿffff82ÿffffacÿffffe2ÿffff84ÿffffa2t know what the function of this unkn
own protein
could be (no similarity to any known protein). However, I
have some
information that may suggest that this protein could work as
a
transcription factor. First, the protein is a small one (124
amino
acids). Second, it is a basic protein, the theoretical pI is
8-9
(depending on the software used). And finally, using the
MatchMaker
two-hybrid system, when the hybrid DNA-BD/Protein A was
expressed in
yeast, it stimulated by itself the reporter genes tran
scription. I have
heard about the new CytoTrap two-hybrid system, which could
be suitable
for my case. In addition, I want to address the subcellular
l
ocation of
protein A. I wonder whether there is any other
straightforward
technique to test my hypothesis (protein A is a
transcription
factor). I
would appreciate any thought on this.
Thanks in advance
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