IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

want to know method which can effeciently lysis cultured cells wh ile keeping the acitivity of

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Wed Jan 12 10:22:25 EST 2000


In article <22E71DAEC504D111B78100805FFE9DC72E68715E at pfs21.ex.nus.edu.sg>, Li Jingsong <medp7095 at nus.edu.sg> wrote:
>Hello, 
>
>  Anyone can tell me an efficient method to lysis cultured cells while
>keeping the activity of cAMP dependent
>  Protein kinase?
>
>  Thanks 

I think PKA is quite happy in mild detergents. Thus, just dissolve 
the cells in 0.5-1% of something like octylglucoside, Triton, tween,
etc - depending on your application. 

        - Dima




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net