In article <22E71DAEC504D111B78100805FFE9DC72E68715E at pfs21.ex.nus.edu.sg>, Li Jingsong <medp7095 at nus.edu.sg> wrote:
>> Anyone can tell me an efficient method to lysis cultured cells while
>keeping the activity of cAMP dependent
> Protein kinase?
I think PKA is quite happy in mild detergents. Thus, just dissolve
the cells in 0.5-1% of something like octylglucoside, Triton, tween,
etc - depending on your application.