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want to know method which can effeciently lysis cultured cells wh ile keeping the acitivity of

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Wed Jan 12 10:22:25 EST 2000

In article <22E71DAEC504D111B78100805FFE9DC72E68715E at pfs21.ex.nus.edu.sg>, Li Jingsong <medp7095 at nus.edu.sg> wrote:
>  Anyone can tell me an efficient method to lysis cultured cells while
>keeping the activity of cAMP dependent
>  Protein kinase?
>  Thanks 

I think PKA is quite happy in mild detergents. Thus, just dissolve 
the cells in 0.5-1% of something like octylglucoside, Triton, tween,
etc - depending on your application. 

        - Dima

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