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DNA isolation

Teresa Wilson Teresa.Wilson at dpiwe.tas.gov.au
Wed Jan 12 21:59:36 EST 2000


A really fast way is just to add 100 µl (40mg/mL) lysozyme per 1ml
suspension of bacteria. Incubate for 1 hour at 37°C, then boil for
15min, cool in ice for 5 min and spin collecting the supernatant.

Regards T
On Fri, 7 Jan 2000 19:33:55 +0800, "Chong Wai Yin( Zhang Weixian)"
<wychong at singnet.com.sg> wrote:

>Hi, does anyone know of any fast, cheap and dirty method to extract DNA from
>a Gram positive bacteria? Thanks.
>
>Kelvin.
>
>





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