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Guanidine HCl and plasmid preps

Teresa Wilson Teresa.Wilson at dpiwe.tas.gov.au
Wed Jan 12 22:33:46 EST 2000

I use,: Rapid and simple method for purification of nucleic acids. R.
Boom et al. Journal of Clinical Microbiology. Mar. 1990 p. 495-503.
for extracting my DNA it waorks well. Be careful with the TE if your
using high template volumes as the EDTA can inhibit PCR.

On Wed, 12 Jan 2000 14:10:07 +0000, Simon Dawson
<Simon.Dawson at nottingham.ac.uk> wrote:

>Hi all,
>        Does anyone have any methods (including buffer ingredients etc) for
>modified alkaline lysis plasmid minipreps that use guanidine HCl in the
>neutralising solution and bind plasmid DNA to silica prior to elution in TE?
>    Thanks in advance.
>            Simon Dawson
>Dr. Simon Dawson
>Floor D
>School of Biomedical Sciences
>Queen's Medical Centre
>Clifton Boulevard
>NG7 2UH
>TEL:+44 (0)115 9709419          FAX:+44 (0)115 9709969
>Email:Simon.Dawson at nott.ac.uk
>"Back off man, I'm a scientist!" - Bill Murray.

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