I am desperately trying to find back a reference where I read about people
that used lambda exonuclease to generate single strand ends (about 300 bp)
on linearized plasmids and used these to transfect cells. I remembered I had
the paper by accident and that there was actually no mention of lambda
exonuclease in the abstract. If anyone knows of this paper or a similar one,
please email me...
Thanks a lot.
erheaume at procrea.qc.ca