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Looking for a reference where lambda exonuclease was used to generate single strand linker at plasmid ends

Ying Jiang yijst1 at pitt.edu
Thu Jan 13 19:48:36 EST 2000


i don't know what you are looking for. but i have just read a paper on cloning
using lambda exonuclease: H. Tseng, DNA cloning without restriction enzyme and
ligase. BioTechniques 27: 1240-1244 (Dec 1999)
wish it can help. Good luck

Eric Rheaume wrote:

> Hi!
>
> I am desperately trying to find back a reference where I read about people
> that used lambda exonuclease to generate single strand ends (about 300 bp)
> on linearized plasmids and used these to transfect cells. I remembered I had
> the paper by accident and that there was actually no mention of lambda
> exonuclease in the abstract. If anyone knows of this paper or a similar one,
> please email me...
>
> Thanks a lot.
>
> Eric Rheaume
> erheaume at procrea.qc.ca
>
> ---





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