IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Nonidet P40 and Bradford

Jim Kami jakami at ucdavis.edu
Thu Jan 13 20:26:01 EST 2000


Nonidet P40  =  IGEPAL-CA = Triton X-100 = Octoxynol - 9 (common
spermatocide)
They are all trade names for the same compound.
ref.  Merck Index  ed. 10  #6601

Jim Kami
Blue Rose Biotech

"Dr. Fernando Rodriguez-Pascual" wrote:

> Hi all,
>
> Have any of you experienced problems in performing protein
> determinations by Bradford using extracts containing 0.5% Nonidet P40. I
> mean, whether it is compatible with the Bradford reagent and up to which
> concentration. In former times I used to have no problems for that but
> now I find that anyhow it affects the assay.
> By the way, Sigma is not longer purchasing Nonidet P40, but a
> "chemically indistinguible from it" compound (as Sigma) called IGEPAL-CA
> 630. Do you know if it is really the same? Have you found problems by
> using this new nonionic detergent?
>
> Chao
>
> Fernando
> --
> #############################################
> Dr. Fernando Rodríguez Pascual
> Department of Pharmacology
> Johannes Gutenberg University
> Obere Zahlbacher Str. 67
> D-55101 Mainz, Germany
> Tf. +49 (6131) 39 3 68 36 or 39 3 32 45
> Fax. +49 (6131) 39 3 66 11
> email: frodrigu at mail.uni-mainz.de
> #############################################





More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net