BSA removal

[M. Johan Broekman]

Nick Theodorakis wrote:
> 
> In article <387DF82A.BD3060FD at cajal.mbb.ki.se>, jonas at cajal.mbb.ki.se
> (Jonas Anders) wrote:
> > Hi all,
> > is there a straightforward technique to remove BSA as main
> > contaminant
> > from the desired target protein (computed pI = 9)
> > Any suggestions highly appreciated
> > Thanx
> > Jonas
> > ---
> 
> I seem to recall that in the old days people used to use Blue-Sepharose
> (Cibaron Blue?) to remove albumin from serum, since the albumin bound
> very tightly to it but the IgG did not.
> 
> Nick
> 
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Cibachron/m Blue is NOT specific for albumin.  It also binds
nucleotide-binding proteins, including heatshock proteins.
-Han