replication of plasmids
srohrerNOSPAM at immv.unizh.ch
Fri Jan 14 05:23:14 EST 2000
so I have gotten 2 answers so far - one says yes, one says no...
> > My question: can a plasmid containing 2 identical origins replicate,
> No, it can't.
> > i.e. could pUC be ligated with itself?
> Yes, it can, but the product will not rreplicate.
"...I think you can do it. I used to study plasmid DNA replication and we
cloned low-copy number plasmid oris into pBR322. All that happens is that
the higher copy number ori becomes dominant. so two identical oris on one
plasmid should be no problem I guess."
More votes anyone?
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