If you haven't already done the recommended titration of G418 yourself,
I did a titration myself just the other week... In my experience I
needed 1 mg/ml G418 to effectively kill off 3T3 cells.
jhjeon2 at snu.ac.kr wrote:
> Hi!! happy new year everyone.
> I want to know appropriate G418 conc. in NIH3T3 transfection
> Let me know it.
>> Thanks in advance.
>> ---
--
Ulf Andersson
Cell and Molecular Biology/Ward 3-334
Northwestern University
303 East Chicago Avenue
Chicago, IL 60611
Phone: 1 (312) 503 4075
Fax: 1 (312) 503 7912