In article <387E7D7C.855B735E at csi.com>, "M. Johan Broekman"
<hanbroekman at csi.com> wrote:
> Nick Theodorakis wrote:
> > In article <387DF82A.BD3060FD at cajal.mbb.ki.se>,
>jonas at cajal.mbb.ki.se> > (Jonas Anders) wrote:
> > > Hi all,
> > > is there a straightforward technique to remove BSA as main
> > > contaminant
> > > from the desired target protein (computed pI = 9)
> > > Any suggestions highly appreciated
> > > Thanx
> > > Jonas
> > > ---
> > I seem to recall that in the old days people used to use
> > (Cibaron Blue?) to remove albumin from serum, since the albumin
> > very tightly to it but the IgG did not.
> > Nick
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> Cibachron/m Blue is NOT specific for albumin. It also binds
> nucleotide-binding proteins, including heatshock proteins.
I didn't say it was specific for albumin, just that it bound it very
tightly. If Jonas's protein does not bind to it, it might be worth a
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