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BSA removal

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Jan 14 10:49:17 EST 2000


In article <387E7D7C.855B735E at csi.com>, "M. Johan Broekman"
<hanbroekman at csi.com> wrote:
> Nick Theodorakis wrote:
> >
> > In article <387DF82A.BD3060FD at cajal.mbb.ki.se>,
> jonas at cajal.mbb.ki.se
> > (Jonas Anders) wrote:
> > > Hi all,
> > > is there a straightforward technique to remove BSA as main
> > > contaminant
> > > from the desired target protein (computed pI = 9)
> > > Any suggestions highly appreciated
> > > Thanx
> > > Jonas
> > > ---
> >
> > I seem to recall that in the old days people used to use
> Blue-Sepharose
> > (Cibaron Blue?) to remove albumin from serum, since the albumin
> bound
> > very tightly to it but the IgG did not.
> >
> > Nick
> >
> > * Sent from RemarQ http://www.remarq.com The Internet's
> Discussion Network *
> > The fastest and easiest way to search and participate in Usenet
> - Free!
> Cibachron/m Blue is NOT specific for albumin.  It also binds
> nucleotide-binding proteins, including heatshock proteins.
> -Han


I didn't say it was specific for albumin, just that it bound it very
tightly. If Jonas's protein does not bind to it, it might be worth a
try.

Nick



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