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Touchdown PCR

Christer Ericsson ericsson at acsu.buffalo.edu
Fri Jan 14 19:02:10 EST 2000


Is there a "standard" protocol for Touchdown PCR?

Apart from the original NAR paper by RH. Don and colaborators, what is the
essential reading?

Any general advice before I do my first experiment with a set of
degenerate primers?

Any advice greatly appreciated.

Will offer to summarize to the group if there is interest.

Sincerely,

Christer Ericsson




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