BSA removal

Warren watchcity at my-deja.com
Fri Jan 14 22:26:39 EST 2000


Hi, DEAE binds albumin, if your target protein doesn't (or elutes @ different
[salt]).  Do you know about their respective sizes? Does your taget protein
have a specific ligand? Alas, pI doesn't tell all.  Happy New Year,  Warren

In article <25d0e1ac.4032302f at usw-ex0104-028.remarq.com>,
  Nick Theodorakis <nicholas_theodorakis at urmc.rochester.edu> wrote:
> In article <387E7D7C.855B735E at csi.com>, "M. Johan Broekman"
> <hanbroekman at csi.com> wrote:
> > Nick Theodorakis wrote:
> > >
> > > In article <387DF82A.BD3060FD at cajal.mbb.ki.se>,
> > jonas at cajal.mbb.ki.se
> > > (Jonas Anders) wrote:
> > > > Hi all,
> > > > is there a straightforward technique to remove BSA as main
> > > > contaminant
> > > > from the desired target protein (computed pI = 9)
> > > > Any suggestions highly appreciated
> > > > Thanx
> > > > Jonas
> > > > ---
> > >
> > > I seem to recall that in the old days people used to use
> > Blue-Sepharose
> > > (Cibaron Blue?) to remove albumin from serum, since the albumin
> > bound
> > > very tightly to it but the IgG did not.
> > >
> > > Nick
> > >
> > > * Sent from RemarQ http://www.remarq.com The Internet's
> > Discussion Network *
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> > Cibachron/m Blue is NOT specific for albumin.  It also binds
> > nucleotide-binding proteins, including heatshock proteins.
> > -Han
>
> I didn't say it was specific for albumin, just that it bound it very
> tightly. If Jonas's protein does not bind to it, it might be worth a
> try.
>
> Nick
>
> * Sent from RemarQ http://www.remarq.com The Internet's Discussion Network *
> The fastest and easiest way to search and participate in Usenet - Free!
>
>


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