chylamidia penumoniae

Tjaco Ossewaarde jm.ossewaarde at hccnet.nl
Sat Jan 15 10:59:59 EST 2000


Hi,

Prot K concentration; 100 - 400 mcg/ml
Currently we have good results using kits from invitrogen (DNA as well as
RNA).

Tjaco
S Hopkirk <s.hopkirk at virgin.net> wrote in message
news:38803794.4EB7DB71 at virgin.net...
> Hi, thanks for your suggestion.  What concentration of prot K do you use
and
> what commercial kit(s) would you recommend?
>
> Thanks,
> Sarah
>
> Tjaco Ossewaarde wrote:
>
> > In our experience, the use of some kind of protein degrader (like prot
K) is
> > essential. Next, various methods can be used for purification, including
> > commercial kits.
> >
> > Tjaco
> > S Hopkirk <s.hopkirk at virgin.net> wrote in message
> > news:387E4117.27AA136D at virgin.net...
> > > What methods has anyone used to extract genomic DNA from Chyamydia
> > > pneumoniae cells?
> > >
> > > I have tried with limited success using a Phenol Chloroform method.
Is
> > > there a better way?
> > >
> > > Thanks,
> > >
> > > Sarah
> > >
>





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