overexpression in neurons

Jan Behrends j.behrends at lrz.uni-muenchen.de
Sat Jan 15 15:19:56 EST 2000


A (probably naive) question:

I'd like to overexpress a 525AA protein in primary cell culture of neurons.
I am not a molecular biologist (and I intend to stay that way if poss.) but
have heard that nowadays you can have a vector (like herpes simplex) custom
made by a biotech firm and then use it to infect the cells and make them
overexpress whatever you want.

What's the procedure and where can I find a firm that will do that?

Any info (if possible by e-mail) is greatly appreciated.
Thanks,

Jan

--
Jan C. Behrends, MD
Department of Physiology
University of Munich
Pettenkoferstr. 12
80336 Muenchen
Germany
Phone: (49-/0-) 89 5996 248/251
Fax: (49-/0-) 89 5996 216
e-mail: j.behrends at lrz.uni-muenchen.de





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