chylamidia penumoniae

S Hopkirk s.hopkirk at virgin.net
Sun Jan 16 17:35:43 EST 2000


Thank you  for your help, I will now try to put theory into practise!

Sarah

Tjaco Ossewaarde wrote:

> Hi,
>
> Prot K concentration; 100 - 400 mcg/ml
> Currently we have good results using kits from invitrogen (DNA as well as
> RNA).
>
> Tjaco
> S Hopkirk <s.hopkirk at virgin.net> wrote in message
> news:38803794.4EB7DB71 at virgin.net...
> > Hi, thanks for your suggestion.  What concentration of prot K do you use
> and
> > what commercial kit(s) would you recommend?
> >
> > Thanks,
> > Sarah
> >
> > Tjaco Ossewaarde wrote:
> >
> > > In our experience, the use of some kind of protein degrader (like prot
> K) is
> > > essential. Next, various methods can be used for purification, including
> > > commercial kits.
> > >
> > > Tjaco
> > > S Hopkirk <s.hopkirk at virgin.net> wrote in message
> > > news:387E4117.27AA136D at virgin.net...
> > > > What methods has anyone used to extract genomic DNA from Chyamydia
> > > > pneumoniae cells?
> > > >
> > > > I have tried with limited success using a Phenol Chloroform method.
> Is
> > > > there a better way?
> > > >
> > > > Thanks,
> > > >
> > > > Sarah
> > > >
> >





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