replication of plasmids

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Mon Jan 17 03:31:41 EST 2000


Robert Whittier wrote:

> Frank O. Fackelmayer wrote:
>
> >>Plasmids transformed into recA+ strains naturally form dimers and trimers,
> >>and I have a vague recollection of some paper in the late 70's that showed
> >>you could isolate the monomer band or the dimer band. Transformed back
> >>into a recA- strain, each would replicate faithfully in whichever form
> >>(monomer or dimer) was used to transform. Transformed back into a recA+
> >>strain, the same spectrum of monomer, dimer, trimer, etc. forms would
> >>reappear.
> >>
> >
> >could you try to dig out the reference? That sounds interesting and I would
> >like to see the original data...
> >
> >Frank
>
> Since PubMed doesn't include abstracts for papers going this far back,
> I had to make a special trip from my home to the Tsukuba University
> Medical School library to dig this up, but here you go, Frank.
>
> Potter, H. and Dressler, D. (1977) On the mechanism of genetic
> recombination: the maturation of recombination intermediates.
> Proc. Natl. Acad. Sci. USA 74: 4168-4172.
>
> It was really elegant work in view of the state of the art at that
> time. Other workers followed it up to investigate various genetic
> requirements. For example:
>
> James, A.A., Morrison, P.T. and Kolodner, R. (1982) Genetic
> recombination of bacterial plasmid DNA; analysis of the effect of
> recombination-deficient mutation on plasmid recombination. J. Mol.
> Biol. 160: 411-430.
>

Thanks!
Frank







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