Transformation problems...

Andrew Leung s970854 at cuhk.edu.hk
Mon Jan 17 06:07:40 EST 2000


Hi,
    Probably competent cells didn't work. Is there any strong radioactive
present in your department freezer? Maybe the competent cells die in the
freezer. Try freshly prepared competent cells.

Andrew


khalil ¼¶¼g©ó¤å³¹ ...
>Hi everyone !
>
>I am writing on behalf of an entire departement of microbiology which
>students are loosing all hopes. We are at least 7 students in 5 different
>laboratories of the university who are not able to have a single colonie
>after transformation.
>We would like to know if anyone ever encountered such a problem. Please
>avoid any joke about our ability to transform, we all have either master's
>or PhD. This week we decided to look at what we did in common in our
>experiments that would be the cause of our problems. The only thing that we
>found would be the water we use (millipore milli-Q). We all get our
>deionised water from the same source in the departement. Could it be the
>cause, a problem with the filters or anything ? while we are trying to
>find-out, any help would be greatly appreciated. Did anyone ever had
>problems of transformation due to water. Thank you in advance.
>
>






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