All possible methods are being used, we all work in different labs in the
departement.
In ours we use the CaCl2 method and a new method involving TSS. Competent
cells are different for each one and stored in different freezers. We don't
even use the same strain of E.coli.
"Kiley R. Prilliman" <tiey at aol.comjunkbloc> wrote in message
news:20000117030214.10870.00000733 at ng-fg1.aol.com...
> Why don't you tell us a little about the method you all are using? That
would
> help us out in addressing any problem(s)...
>> best,
> Kiley
>>> Kiley R. Prilliman, Ph.D.
> Postdoctoral Research Fellow
> Division of Immune Regulation
> La Jolla Institute for Allergy & Immunology
>>kiley at liai.org