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Transformation problems...

Jan-Henner Wurmbach wurmbach at uke.uni-hamburg.de
Mon Jan 17 10:40:04 EST 2000


"R. Jayakumar" <jakku at mrna.tn.nic.in> schrieb im Beitrag
<004601bf6031$e485c820$8c01a8c0 at tn.nic.in>...
[snip]

>     ONE STEP TRANSFORMATION PROTOCOL FOR E.COLI
[snip ]
> 5. Take a 100ul aliquot and add DNA (around 10-20ng) and leave on ice for
> 30min (we found that heat shock treatment gives less transformants than
ice
> treatment).
Interesting! In our lab Heat shock is needed, at least with ligations. For
supercoiled Plasmids there seems to be no difference. Maybe plenty of DNA
and HS is giving an efficiency that is inhibiting?

Greetings 
  Jan-Henner Wurmbach <wurmbach at uke.uni-hamburg.de>

[snip rest of mail]




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