Transformation problems...

[Susanne Rohrer]

"Richard P. Grant" wrote:

> Milli-Q water is 'clinical' grade.  At least, I know they used to use it
> to incubate the eggs in an IVF unit.

exactly. If it's pure enough for cell culture, its fine for bacteria.
Maybe the guys should check their Millipore unit for contamination - It
happened in my previous lab, and it really messed up their cell culture.

Another suggestion: Are you sure (although you're using several different
strains) - that the bacteria are still what you think they are? Why not get
another strain from a friendly lab?