Transformation problems...

Susanne Rohrer srohrerNOSPAM at immv.unizh.ch
Mon Jan 17 12:12:30 EST 2000



"Richard P. Grant" wrote:

> Milli-Q water is 'clinical' grade.  At least, I know they used to use it
> to incubate the eggs in an IVF unit.

exactly. If it's pure enough for cell culture, its fine for bacteria.
Maybe the guys should check their Millipore unit for contamination - It
happened in my previous lab, and it really messed up their cell culture.

Another suggestion: Are you sure (although you're using several different
strains) - that the bacteria are still what you think they are? Why not get
another strain from a friendly lab?





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