Shearing DNA

Muhammad A. Lodhi mlodhi at biocept-inc.com
Mon Jan 17 17:59:47 EST 2000


Hi All,

I am trying to chop up human genomic DNA or cDNA randomly into small
fragments of 50-150 bases.  This DNA is in up to 500 uL of TE.  Can
someone please suggest me what is the best way of doing so among
nebulization, sonication and restriction digest (with which
enzyme(s)?).  I need this fragmented DNA in some hybridization analyses.

Thanks
Muhammad A. Lodhi

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