Try grinding sample in liquid nitrogen then add 5x concentrated SDS sample
loading buffer (without DTT or BME) and boil for a few min, cool to RT then
add the BME or DTT and centrifuge to remove the particulates. Samples should
be prepared as required and not stored.
"Axel Schwekendiek" <schwekdk at pz.uni-hohenheim.de> wrote in message
news:387E1A15.DE70DBD0 at pz.uni-hohenheim.de...
> does anybody know a protocol for preparing protein from potato tubers
> for use in western blots ?
>> Thanks !