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Potato tuber protein

Andrew Liddell AndrewLiddell at nospam.Hotmail.com
Mon Jan 17 18:22:01 EST 2000


Try grinding  sample in liquid nitrogen then add 5x concentrated SDS sample
loading buffer (without DTT or BME) and boil for a few min, cool to RT then
add the BME or DTT and centrifuge to remove the particulates. Samples should
be prepared as required and not stored.

"Axel Schwekendiek" <schwekdk at pz.uni-hohenheim.de> wrote in message
news:387E1A15.DE70DBD0 at pz.uni-hohenheim.de...
> does anybody know a protocol for preparing protein from potato tubers
> for use in western blots ?
>
> Thanks !
>
> ---






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