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Rehydration of DNA

JCarson jeremy.carson at dpiwe.tas.gov.au
Tue Jan 18 02:27:35 EST 2000


We have noted that DNA eluted from silica with 10mM pH8 TRIS buffer
does not amplify well by PCR if the  DNA is used immediately after
elution. If the eluted DNA is stored at 4C overnight and used in a PCR
then good amplification occurs. 
We presume that the poor amplification that occus immediately after
elution is because the DNA is not fully rehydrated.
Any comments please about what is the nature of DNA rehydration. Why
would a PCR not work well if the DNA is not adequately rehydrated? Is
it likely that genomic DNA with high (or low) G+C content may
rehydrate faster (or slower). Any suggestions as to how to speed up
rehydration apart from raising the pH or warming the elution buffer.
Any comments observations?
Thanks

Jeremy Carson
Dept Primary Industry, Tasmania
Australia
jeremy.carson at dpiwe.tas.gov.au




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