Rehydration of DNA

Richard P. Grant rgrant at netscape.net
Tue Jan 18 03:45:06 EST 2000


In article <388411aa.23358787 at news.tas.gov.au>, 
jeremy.carson at dpiwe.tas.gov.au (JCarson) wrote:

> We presume that the poor amplification that occus immediately after
> elution is because the DNA is not fully rehydrated.

It is probably more likely that there is silica in your DNA prep, and 
that overnight it settles on the bottom of the tube.  Silica inhibits 
PCR.

Trust me on this :-))

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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