Shearing DNA

Jan-Henner Wurmbach wurmbach at uke.uni-hamburg.de
Tue Jan 18 05:46:28 EST 2000


You want random Fragments?
Autoclaving works well, at least with Salmon Sperm...

"Muhammad A. Lodhi" <mlodhi at biocept-inc.com> schrieb im Beitrag
<38839EC3.6031F576 at biocept-inc.com>...
> Hi All,
> 
> I am trying to chop up human genomic DNA or cDNA randomly into small
> fragments of 50-150 bases.  This DNA is in up to 500 uL of TE.  Can
> someone please suggest me what is the best way of doing so among
> nebulization, sonication and restriction digest (with which
> enzyme(s)?).  I need this fragmented DNA in some hybridization analyses.
> 
> Thanks
> Muhammad A. Lodhi
> 
> ---
> 




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