"Muhammad A. Lodhi" wrote:
> Hi All,
>> I am trying to chop up human genomic DNA or cDNA randomly into small
> fragments of 50-150 bases. This DNA is in up to 500 uL of TE. Can
> someone please suggest me what is the best way of doing so among
> nebulization, sonication and restriction digest (with which
> enzyme(s)?). I need this fragmented DNA in some hybridization analyses.
If you want a random collection I would say that sonication is the easiest
way. You'll have to determine though whether the range of the distribution
is adequate for your purposes. It can be rather broad.