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Shearing DNA

Colin Rasmussen colin at pombe.usask.ca
Tue Jan 18 11:51:23 EST 2000



"Muhammad A. Lodhi" wrote:

> Hi All,
>
> I am trying to chop up human genomic DNA or cDNA randomly into small
> fragments of 50-150 bases.  This DNA is in up to 500 uL of TE.  Can
> someone please suggest me what is the best way of doing so among
> nebulization, sonication and restriction digest (with which
> enzyme(s)?).  I need this fragmented DNA in some hybridization analyses.

If you want a random collection I would say that sonication is the easiest
way.  You'll have to determine though whether the range of the distribution
is adequate for your purposes.  It can be rather broad.

Colin





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