IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

pfu and Taq PCR mutation percentage

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Jan 19 04:26:55 EST 2000

In article <862t5t$4mi$1 at ssauraab-i-1.production.compuserve.com>, Gys de
Jongh <GysdeJongh at compuserve.com> writes
>did not yet look up Duncan's references because I like to have some fun with
>the problem myself first.

New addition sent to me by e-mail and hopefully she will join this
thread with some answers.

>From: "Janice Cline" <janice_cline at stratagene.com>
>Sender: "Janice Cline" <janice_cline at stratagene.com>
>Reply-To: "Janice Cline" <janice_cline at stratagene.com>
>Importance: normal
>Priority: normal
>Subject: Re[2]: pfu and Taq PCR mutation percentage
>Message-Id: <931406502 at stratagene.com>
>X-MIME-Engine: v0.90
>MIME-Version: 1.0
>Content-Type: text/plain; charset=US-ASCII
>Content-Id: <931406502-1 at stratagene.com>
>Content-Transfer-Encoding: 7bit

>I don't quite understand the problem you have with our error rate
>equation:   ER = mf/(b x d)  where d is calculated as you've stated
>below.  I am not a math whiz, but our results are reproducible.
>Thanks,  janice cline  


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net