pfu and Taq PCR mutation percentage

Dr. Duncan Clark Duncan at
Wed Jan 19 04:26:55 EST 2000

In article <862t5t$4mi$1 at>, Gys de
Jongh <GysdeJongh at> writes
>did not yet look up Duncan's references because I like to have some fun with
>the problem myself first.

New addition sent to me by e-mail and hopefully she will join this
thread with some answers.

>From: "Janice Cline" <janice_cline at>
>Sender: "Janice Cline" <janice_cline at>
>Reply-To: "Janice Cline" <janice_cline at>
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>Priority: normal
>Subject: Re[2]: pfu and Taq PCR mutation percentage
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>I don't quite understand the problem you have with our error rate
>equation:   ER = mf/(b x d)  where d is calculated as you've stated
>below.  I am not a math whiz, but our results are reproducible.
>Thanks,  janice cline  


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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