pfu and Taq PCR mutation percentage
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Jan 19 04:26:55 EST 2000
In article <862t5t$4mi$1 at ssauraab-i-1.production.compuserve.com>, Gys de
Jongh <GysdeJongh at compuserve.com> writes
>did not yet look up Duncan's references because I like to have some fun with
>the problem myself first.
New addition sent to me by e-mail and hopefully she will join this
thread with some answers.
>From: "Janice Cline" <janice_cline at stratagene.com>
>Sender: "Janice Cline" <janice_cline at stratagene.com>
>Reply-To: "Janice Cline" <janice_cline at stratagene.com>
>Subject: Re: pfu and Taq PCR mutation percentage
>Message-Id: <931406502 at stratagene.com>
>Content-Type: text/plain; charset=US-ASCII
>Content-Id: <931406502-1 at stratagene.com>
>I don't quite understand the problem you have with our error rate
>equation: ER = mf/(b x d) where d is calculated as you've stated
>below. I am not a math whiz, but our results are reproducible.
>Thanks, janice cline
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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