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Protein dephosphorylation for Western?

Petri Kerokoski kerokosk at mail.uku.fi
Wed Jan 19 17:58:45 EST 2000


I have an antibody that recognizes two spesific looking bands in western.
The protein of interest is possibly phosphorylated. I know that it is
possible to dephosphorylate the proteins before running the gel to
determine if a band is produced by a phosphorylated form of the studied
protein. I would appreciate if somebody could send me a protocol for the
dephosphorylation reaction. 

Petri Kerokoski

Petri Kerokoski
University of Kuopio
Dept of Neuroscience and Neurology/ Canthia
PO Box 1627
70211 Kuopio

e-mail: petri.kerokoski at uku.fi
tel: +358-17-162554, +358-17-162686
fax: +358-17-162048

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