gain of function mutagenesis, how to do?

Richard P. Grant rgrant at netscape.net
Wed Jan 19 10:09:39 EST 2000


In article <3885C81B.28540D0 at gmx.net>, Frederik Börnke 
<ricky_boernke at gmx.net> wrote:

> Actually, I did some deletion constructs. However, if I delete only
> small
> portions from either C or N terminus interaction is completely
> abolished.
> What does that tell me wiht respect to the isoform speceficity of inter
> action?

I don't know what it tells you about isoform specificity, but how did 
you determine what deletions to make?  Are you sure that your mutants 
are folded?

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk




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