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How to design scrambled oligos?

Colin Rasmussen colin at pombe.usask.ca
Wed Jan 19 11:05:51 EST 2000

Zhonglin Chai wrote:

> Dear All, May I ask how you people usually design your scrambled oligos to
> be used as antisense oligo controls? Are there any rules to follow? Do you
> do it manually or by a computer program?

I roll die.  1 = G, 2 = A, 3 = T, 4 = C.  If I roll 5 or 6 I just roll
again.  Once I've run out of a nucleotide, this also becomes roll again.
There might be faster ways, but this is so much more fun.

Colin Rasmussen

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