gain of function mutagenesis, how to do?

Frederik Börnke ricky_boernke at gmx.net
Wed Jan 19 11:28:21 EST 2000



"Richard P. Grant" schrieb:
> 
> In article <3885C81B.28540D0 at gmx.net>, Frederik Börnke
> <ricky_boernke at gmx.net> wrote:
> 
> > Actually, I did some deletion constructs. However, if I delete only
> > small
> > portions from either C or N terminus interaction is completely
> > abolished.
> > What does that tell me wiht respect to the isoform speceficity of inter
> > action?
> 
> I don't know what it tells you about isoform specificity, but how did
> you determine what deletions to make?  Are you sure that your mutants
> are folded?
> 
> R

I am not sure whether they are folded properly, how could I determine?

also R


> Richard P. Grant MA DPhil
> Structural Studies Group, MRC-LMB
> http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
> Please reply to rpg 'at' mrc-lmb.cam.ac.uk

-- 
*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
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