production of antisense oligonucleotide by PCR ?

Jacqueline Fischer-Lougheed Jacqueline.Fischer-Lougheed at medecine.unige.ch
Wed Jan 19 11:40:25 EST 2000


Has anyone ever tried to produce large amounts of single-stranded
oligonucleotides (about 200 bases long) for ANTISENSE technology using PCR
on a linearized vector?
I thank you in advance for references or protocols (or reasons why this
idea is no good!).

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Jacqueline Fischer-Lougheed, PhD
Physiology Department / C.M.U. (University of Geneva Medical School)
1 rue Michel-Servet
1211 GENEVA 4
Switzerland

tel. (+41) (22) 702.53.95 - fax. (+41) (22) 702.54.02
e-mail:  Jacqueline.Fischer-Lougheed at medecine.unige.ch
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