Colin Rasmussen wrote:
>> Zhonglin Chai wrote:
>> > Dear All, May I ask how you people usually design your scrambled oligos to
> > be used as antisense oligo controls?
>> I roll die. 1 = G, 2 = A, 3 = T, 4 = C. If I roll 5 or 6 I just roll
> again. Once I've run out of a nucleotide, this also becomes roll again.
Or change the system to, eg. 1,2=G 3,4=A 5,6=T.
When you run out of another it becomes, eg. 1,2,3=G 4,5,6=A
This would cut down the number of re-rolls.
> There might be faster ways, but this is so much more fun.
I think it's cool :-)
I think I'll have to bring a six and a four-sided dice into work, just
in case I need to scramble an oligo in the future.
> Colin Rasmussen