How to design scrambled oligos?
AFS7 at le.ac.uk
Wed Jan 19 19:45:12 EST 2000
Colin Rasmussen wrote:
> Zhonglin Chai wrote:
> > Dear All, May I ask how you people usually design your scrambled oligos to
> > be used as antisense oligo controls?
> I roll die. 1 = G, 2 = A, 3 = T, 4 = C. If I roll 5 or 6 I just roll
> again. Once I've run out of a nucleotide, this also becomes roll again.
Or change the system to, eg. 1,2=G 3,4=A 5,6=T.
When you run out of another it becomes, eg. 1,2,3=G 4,5,6=A
This would cut down the number of re-rolls.
> There might be faster ways, but this is so much more fun.
I think it's cool :-)
I think I'll have to bring a six and a four-sided dice into work, just
in case I need to scramble an oligo in the future.
> Colin Rasmussen
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