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direct sequencing of cosmid DNA

Warren Gallin wgallin at gpu.srv.ualberta.ca
Wed Jan 19 19:42:32 EST 2000



Colin Dolphin wrote:

> Dqear All
>
> Has anyone successfully obtained good quality sequence directly from
> cosmid DNA ?
>
>

Yes, two weeks ago, using cosmids constructed with the pWE15 vector and
CsCl purified (although I doubt that is essential), standard T3 and T7
primers, Amersham ET sequencing kit, reaction conditions and processing
as described in the manual that comes with the kit.

Warren Gallin

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