Colin Dolphin wrote:
> Dqear All
>> Has anyone successfully obtained good quality sequence directly from
> cosmid DNA ?
>>
Yes, two weeks ago, using cosmids constructed with the pWE15 vector and
CsCl purified (although I doubt that is essential), standard T3 and T7
primers, Amersham ET sequencing kit, reaction conditions and processing
as described in the manual that comes with the kit.
Warren Gallin
-------------- next part --------------
A non-text attachment was scrubbed...
Name: wgallin.vcf
Type: text/x-vcard
Size: 426 bytes
Desc: Card for Warren Gallin
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/20000119/1bddbed0/wgallin.bin
