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How to design scrambled oligos?

A.F. Simpson AFS7 at le.ac.uk
Thu Jan 20 13:32:30 EST 2000


Richard P. Grant wrote:
> 
> In article <38865A98.2959 at le.ac.uk>, "A.F. Simpson" <AFS7 at le.ac.uk>
> wrote:
> 
> > Or change the system to, eg.  1,2=G  3,4=A  5,6=T.
> 
> There's no 'C' in your DNA then?  :)

It was an example of what to do when one of the bases runs out, as the
example below was of what to do when another base runs out.  It reduces
the number of re-rolls requried as the number of available bases drops. 
Using the original system, by the time you get to 2 bases (not unlikely
in, for example, a very GC rich region) only 1/3 of the rolls produces a
useful result.

> Richard P. Grant

love
Anna




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