cosmid sequencing

Mart Speek smart at kbfi.ee
Thu Jan 20 06:55:06 EST 2000


Colin Dolphin wrote:

> Dear All
>
> Has anyone successfully obtained good quality sequence directly from
> cosmid DNA ?

Yes, thats true also for my cosmid (pWE15, 0.5 microgram) containing
35 kb insert, prepared by standard alkaline miniprep procedure,
and sequenced with T3 & T7 primers and BigDye kit.

Also, I must admit that the sequence is not as clean as you normally
obtain with plasmids, but still it is readable <400-500 bases.

Mart Speek (smart at kbfi.ee in ESTONIA)

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