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blotting of large RNAs

Frederik Börnke ricky_boernke at gmx.net
Thu Jan 20 09:56:32 EST 2000


Hi all,

I'd would like to improve transfer of large RNAs during the blotting
procedure. AFAIK incubation of the gel with NaOH or NH4AC prior to
blotting should help. Has anybody a detailed protocol?

TIA
Ricky



*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de




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